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Recent developments in DNA nanotechnology facilitate a new degree of control to place arbitrary objects. Therefore, DNA is folded into arbitrary 2- and 3-dimensional structures on a nanometer to micrometer scale by self assembly and objects of interest are attached to individual incorporated DNA strands. Based on the vision of complex self-assembled nanosystems, we have immobilized fluorophores and gold nanoparticles on DNA origami structures. We carried out fluorescencelifetime imaging to quantitatively describe the interaction between single fluorophores and the metallic nanoparticles in different arrangements. Our experiments focus on the dependence of the fluorophore- metal nanoparticle interaction with different parameters such as nanoparticle size, distance to the nanoparticle and number of nanoparticles.
Acuna, G. P., et. al. Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami. ACS Nano 2012, online. |
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