| Knowledge of the three-dimensional architecture of macromolecules provides valuable infor¬mation on their function and mechanism. X-ray crystallography has contributed enormously, never¬theless, there remain many issues that need attention. These include X-ray photoreduction of redox sensitive cofactors, casting doubts on the identity of the modelled species, the charac¬te¬ri¬zation of reaction intermediates and pathways, and the interactions of the molecule investigated in its cellular con¬text. These topics need approaches in addition to X-ray crystallography and include spectroscopy, computation, solution scattering and imaging. These issues will be illustrated with studies on photoreceptors. |
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