| The mobility of transcription factors and coregulatory proteins within the nuclear compartment reflects transient interactions with chromatin, as well as network interactions with a variety of protein partners. We are using Förster resonance energy transfer (FRET)-based microscopy approaches to define these network interactions in living cells. These methods are being used to measure the dynamic interactions between the homeodomain transcription factor Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBPα) in the nucleus of living mouse pituitary cells. In addition, we have monitored dynamic interactions between C/EBPα and the heterochromatin protein-1 alpha (HP1α) in regions of the centromeric heterochromatin in the pituitary cells. |
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